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Valiant Co Ltd rabbit anti β galactosidase
Rabbit Anti β Galactosidase, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 292 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+%CE%B2+galactosidase/%CE%B2-Galactosidase/pmc13094369-672-0-3
Average 96 stars, based on 292 article reviews
rabbit anti β galactosidase - by Bioz Stars, 2026-10
96/100 stars

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Related Articles

Incubation:

Article Title: Alternate Grainy head isoforms regulate Drosophila midgut intestinal stem cell differentiation
Article Snippet: .. Midguts were then incubated in the following primary antibodies: chicken anti-GFP (1:2000, AbCam, ab307275), mouse anti-Delta (1:100, DSHB, C594.9B), rabbit anti-Pdm-1 (1:2000, Xiaohang Yang), rabbit anti-pH3 (1:5000, Upstate, now Merck, #06-570), mouse anti-β-Galactosidase (1:20, DSHB), chicken anti-β-Galactosidase (1:2000, AbCam, ab134435), rabbit anti-β-Galactosidase (1:2000, Cappel, now MP Biomedical, #55976), mouse anti-GRH (1:5, Sarah Bray), rat anti-GRH (1:500, Stefan Thor), rabbit anti-GRH (1:200, William McGinnis /1:1000, Melissa Harrison), rabbit anti-Activated Caspase 3 (D175) (1: 200, Cell Signaling Technology,) overnight at 4 °C. .. After primary antibody incubation, the samples were washed 3× for 5 min in PBT and incubated in corresponding Alexa Fluor (1:500, Invitrogen) secondary antibodies for 2 h. This was then followed by a DAPI wash for 20 min and 3× washes in PBT for 5 minutes each.

Article Title: A microbiome-derived olfactory signal regulates inter-male aggression and social dominance in mice
Article Snippet: .. Sections were washed in PBS and then blocked in Blocking Buffer (Blocking Reagent Powder, Akoya Biosciences FP1012, in 1X phosphate buffered saline with 0.18% Triton X-100) and then incubated with rabbit anti-β-galactosidase (1:1000, MP Biomedicals, 08559761) and goat anti-OCAM (1:500, R&D Systems, AF778) in Blocking Buffer overnight at 4°C. .. Sections were washed, incubated with donkey anti-goat AlexaFluor 488 (1:500, Invitrogen, A11055) and horse anti-rabbit, biotinylated (1:500, Vector Labs, BA-1100) in Blocking Buffer for 1 hour, washed again, incubated with Streptavidin, AlexaFluor 647 conjugate (1:300, Invitrogen, S-21374) in Blocking Buffer for 30 mins, washed again, and imaged.

Dominant Negative Mutation:

Article Title: Temporal coordination of tissue transformation, olfactory sensory neural development and central axon projections through morphogens
Article Snippet: .. The primary antibodies used in this study were rabbit anti-β-Galactosidase (08559762, MP Biomedicals), mouse anti-Wg (4D4, Developmental Studies Hybridoma Bank (DSHB)), rat anti-DN-cadherin (DN-Ex#8, DSHB), mouse anti-Bruchpilot (nc82, DSHB), chicken anti-GFP (GFP-1020, Aves Labs), rabbit anti-GFP (A11222, Invitrogen), rat anti-Spitz (Anti-Spitz, DSHB), rat anti-mCD8 (MCD0800, Invitrogen). .. The secondary antibodies used and from Jackson ImmunoResearch Laboratories were goat anti-mouse IgG-Alexa Fluor 488 (115-545-166), goat anti-mouse IgG-CyTM3 (115-165-166), goat anti-mouse IgG-Alexa Fluor 647 (115-605-166), goat anti-rabbit IgG-Alexa Flour 488 (111-545-144), goat anti-rabbit IgG-Cy3 (111-165-144), goat anti rabbit IgG-Alexa Fluor 647 (11-605-144), goat anti-rat IgG-Alexa Fluor 488 (112-545-167), goat anti-rat IgG-CyTM3 (112-165-167), goat anti-rat IgG-Alexa Fluor 647 (112-605-167), goat anti-rat IgG-DyLight 649 (112-495-167), and goat anti-chicken IgY (IgG)-DyLight488 (103-485-155).

Blocking Assay:

Article Title: A microbiome-derived olfactory signal regulates inter-male aggression and social dominance in mice
Article Snippet: .. Sections were washed in PBS and then blocked in Blocking Buffer (Blocking Reagent Powder, Akoya Biosciences FP1012, in 1X phosphate buffered saline with 0.18% Triton X-100) and then incubated with rabbit anti-β-galactosidase (1:1000, MP Biomedicals, 08559761) and goat anti-OCAM (1:500, R&D Systems, AF778) in Blocking Buffer overnight at 4°C. .. Sections were washed, incubated with donkey anti-goat AlexaFluor 488 (1:500, Invitrogen, A11055) and horse anti-rabbit, biotinylated (1:500, Vector Labs, BA-1100) in Blocking Buffer for 1 hour, washed again, incubated with Streptavidin, AlexaFluor 647 conjugate (1:300, Invitrogen, S-21374) in Blocking Buffer for 30 mins, washed again, and imaged.

Saline:

Article Title: A microbiome-derived olfactory signal regulates inter-male aggression and social dominance in mice
Article Snippet: .. Sections were washed in PBS and then blocked in Blocking Buffer (Blocking Reagent Powder, Akoya Biosciences FP1012, in 1X phosphate buffered saline with 0.18% Triton X-100) and then incubated with rabbit anti-β-galactosidase (1:1000, MP Biomedicals, 08559761) and goat anti-OCAM (1:500, R&D Systems, AF778) in Blocking Buffer overnight at 4°C. .. Sections were washed, incubated with donkey anti-goat AlexaFluor 488 (1:500, Invitrogen, A11055) and horse anti-rabbit, biotinylated (1:500, Vector Labs, BA-1100) in Blocking Buffer for 1 hour, washed again, incubated with Streptavidin, AlexaFluor 647 conjugate (1:300, Invitrogen, S-21374) in Blocking Buffer for 30 mins, washed again, and imaged.

Immunohistochemistry:

Article Title: MTG8 interacts with LHX6 to specify cortical interneuron subtype identity.
Article Snippet: All sections were blocked in PBS containing 10% heatinactivated serum (sheep or donkey) (Sigma, St. Louis, MO) and 0.1% Triton X-100 at room temperature for 1 hour. .. Immunohistochemistry was performed with the following primary antibodies: rat anti-GFP IgG2a (1:1000; Nacalai Tesque, Kyoto,Japan, #04404-84); rabbit antiGFP (1:5000; Abcam, UK, #ab290); chicken anti-GFP (1:500; Aves Labs, #GFP-1020); rabbit anti-β-galactosidase (1:2000, MP Biomedicals, # 0856032); goat anti-MTG8 (1:200, Santa Cruz Biotechnology, #sc9737); rabbit anti-MTG16 (1:300, Abcam, UK, #ab33072); rabbit anti-PV (1:1000, Chemicon Millipore, #MAB1572); mouse anti-PV (1:1000, Swant Bellizona Switzerland, #235); rabbit anti-SST (1:200, Peninsula Labs, #T-4103.0050); rat anti-SST (1:500, Chemicon Millipore, #MAB354); rabbit anti-calretinin (1:1000, Swant Bellizona Switzerland, #7697); mouse anti-CR (1:500, Swant Bellizona Switzerland, #6B3); rabbit anti-NPY (1:1000, ImmunoStar, #22940), sheep anti-NPY (1:500; Abcam, UK, # ab6173); rabbit anti-nNOS (1:1000; Immunostar, #24287); rabbit anti-SOX6 (1:500, Abcam #ab30455). ..

Immunostaining:

Article Title: A Prox1 enhancer represses haematopoiesis in the lymphatic vasculature.
Article Snippet: Transcriptional enhancer elements are responsible for orchestrating the temporal and spatial control over gene expression that is crucial for programming cell identity during development.. Here we describe a novel enhancer element that is important for regulating the expression of Prox1 in lymphatic endothelial cells.. This evolutionarily conserved enhancer is bound by key lymphatic transcriptional regulators including GATA2, FOXC2, NFATC1 and PROX1.

Article Title: Transcriptomic landscape of early hair follicle and epidermal development
Article Snippet: Samples were imaged using Lumar.V12 stereomicroscope with 1.2x objective and AxioCam ICc camera (Zeiss, Oberkochen, Germany) and ZEN 2.3 lite software (Zeiss Oberkochen, Germany). .. Following antibodies and reagents were used for immunostaining: rat anti-MAdCAM-1 (1:1000; BD Pharmingen), rat anti-EpCAM (CD326) (1:1000; BD Pharmingen), rabbit anti-β-galactosidase (1:500; MP Biomedicals), chicken anti-GFP (1:500; Abcam), Alexa Fluor 568 (1:500; Abcam), Alexa Fluor 647 (1:500; Invitrogen), Alexa Fluor 488 (1:500; Jackson Immunoresearch) and Hoechst-33342 (1:2000; Invitrogen). ..



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Image Search Results


Inhibition of Numb expression enhanced radiation-induced senescence in intestinal epithelial cells. (A-C) Immunohistochemical staining of intestinal sections and western blotting using intestinal mucosal extracts to assess active caspase-3, γH2AX, and p21 expression on days 3 and 7 after 13 Gy WAI. Representative images are displayed. Scale bar = 100 μm. Quantitative analysis of the western blotting results are presented in the form of statistical bars on the right panel of the corresponding immunohistochemistry images. n = 3 mice in each group. (D) Frozen sections of the intestine were stained for senescence-associated β-galactosidase (SA-β-gal) activity in intestinal epithelial cells on days 3 and 7 following 13 Gy WAI. SA- β-galactosidase levels were quantified via western blotting using intestinal mucosal extracts and are presented on the right panel. n = 3 mice in each group. The values are presented as the means ± SEMs. ** P < 0.01, * P < 0.05.

Journal: Scientific Reports

Article Title: Numb mitigates intestinal epithelial cell senescence induced by radiation through a PLK1-dependent pathway

doi: 10.1038/s41598-026-44793-x

Figure Lengend Snippet: Inhibition of Numb expression enhanced radiation-induced senescence in intestinal epithelial cells. (A-C) Immunohistochemical staining of intestinal sections and western blotting using intestinal mucosal extracts to assess active caspase-3, γH2AX, and p21 expression on days 3 and 7 after 13 Gy WAI. Representative images are displayed. Scale bar = 100 μm. Quantitative analysis of the western blotting results are presented in the form of statistical bars on the right panel of the corresponding immunohistochemistry images. n = 3 mice in each group. (D) Frozen sections of the intestine were stained for senescence-associated β-galactosidase (SA-β-gal) activity in intestinal epithelial cells on days 3 and 7 following 13 Gy WAI. SA- β-galactosidase levels were quantified via western blotting using intestinal mucosal extracts and are presented on the right panel. n = 3 mice in each group. The values are presented as the means ± SEMs. ** P < 0.01, * P < 0.05.

Article Snippet: After blocking with TBST containing 5% nonfat dry milk, specific primary antibodies against Numb (Abcam, Ab220362 ), p53 (Abcam, Ab131442 ), p21 (Santa Cruz, sc-377515), p16 (Abcam, ab211542), γH2AX (Santa Cruz, sc-517348), Cyclin B (Santa Cruz, sc-166210), Plk1 (Abcam, ab12157) and β-galactosidase (CST, #27198) were incubated with the membranes at 4 °C overnight, followed by incubation with the respective secondary antibodies.

Techniques: Inhibition, Expressing, Immunohistochemical staining, Staining, Western Blot, Immunohistochemistry, Activity Assay

Inhibition of Numb promoted mitotic arrest and cellular senescence after radiation in colonic cells. (A) Flow cytometry was utilized to assess the apoptosis of HCT116 cells following 24 h and 72 h of radiation. n = 3 repeats in each group. (B) Flow cytometry was utilized to analyze the cell cycle distribution of colonic HCT116 cells at 24 h and 72 h postradiation. ** P < 0.01 IR versus IR+shNumb group at 24 h, * P < 0.05 IR versus IR+shNumb group at 72 h. n = 3 repeats in each group. (C) Immunofluorescence staining was conducted to evaluate the expression of the DNA damage marker γH2AX after 72 h of radiation. Scale bar = 50 μm. (D) Cell senescence-associated β-gal staining was performed to analyze cell senescence after 72 h of radiation. Scale bar = 50 μm.

Journal: Scientific Reports

Article Title: Numb mitigates intestinal epithelial cell senescence induced by radiation through a PLK1-dependent pathway

doi: 10.1038/s41598-026-44793-x

Figure Lengend Snippet: Inhibition of Numb promoted mitotic arrest and cellular senescence after radiation in colonic cells. (A) Flow cytometry was utilized to assess the apoptosis of HCT116 cells following 24 h and 72 h of radiation. n = 3 repeats in each group. (B) Flow cytometry was utilized to analyze the cell cycle distribution of colonic HCT116 cells at 24 h and 72 h postradiation. ** P < 0.01 IR versus IR+shNumb group at 24 h, * P < 0.05 IR versus IR+shNumb group at 72 h. n = 3 repeats in each group. (C) Immunofluorescence staining was conducted to evaluate the expression of the DNA damage marker γH2AX after 72 h of radiation. Scale bar = 50 μm. (D) Cell senescence-associated β-gal staining was performed to analyze cell senescence after 72 h of radiation. Scale bar = 50 μm.

Article Snippet: After blocking with TBST containing 5% nonfat dry milk, specific primary antibodies against Numb (Abcam, Ab220362 ), p53 (Abcam, Ab131442 ), p21 (Santa Cruz, sc-377515), p16 (Abcam, ab211542), γH2AX (Santa Cruz, sc-517348), Cyclin B (Santa Cruz, sc-166210), Plk1 (Abcam, ab12157) and β-galactosidase (CST, #27198) were incubated with the membranes at 4 °C overnight, followed by incubation with the respective secondary antibodies.

Techniques: Inhibition, Flow Cytometry, Immunofluorescence, Staining, Expressing, Marker

PLK1 overexpression compensated for the Numb knockdown-induced cellular senescence phenotype in colonic cells after radiation. (A) Senescence-associated β-gal staining was performed on different groups of HCT116 cells. The results of the quantitative analysis of the percentage of cells with positive β-gal staining are shown in the right panel. Scale bar = 50 μm. (B) Colony formation assay illustrating colonies in different groups of HCT116 cells after radiation. Quantitative analysis of the total optical density of the colony formation assay is shown in the right panel. (C-E) RT‒PCR was used to detect the expression of the indicated cytokines (IL-6, IL-8, and IL-1β) in different groups of cells. The expression levels were normalized to those of β-actin. The values are presented as the means ± SEMs. ** P < 0.01, * P < 0.05. n = 3 repeats in each group.

Journal: Scientific Reports

Article Title: Numb mitigates intestinal epithelial cell senescence induced by radiation through a PLK1-dependent pathway

doi: 10.1038/s41598-026-44793-x

Figure Lengend Snippet: PLK1 overexpression compensated for the Numb knockdown-induced cellular senescence phenotype in colonic cells after radiation. (A) Senescence-associated β-gal staining was performed on different groups of HCT116 cells. The results of the quantitative analysis of the percentage of cells with positive β-gal staining are shown in the right panel. Scale bar = 50 μm. (B) Colony formation assay illustrating colonies in different groups of HCT116 cells after radiation. Quantitative analysis of the total optical density of the colony formation assay is shown in the right panel. (C-E) RT‒PCR was used to detect the expression of the indicated cytokines (IL-6, IL-8, and IL-1β) in different groups of cells. The expression levels were normalized to those of β-actin. The values are presented as the means ± SEMs. ** P < 0.01, * P < 0.05. n = 3 repeats in each group.

Article Snippet: After blocking with TBST containing 5% nonfat dry milk, specific primary antibodies against Numb (Abcam, Ab220362 ), p53 (Abcam, Ab131442 ), p21 (Santa Cruz, sc-377515), p16 (Abcam, ab211542), γH2AX (Santa Cruz, sc-517348), Cyclin B (Santa Cruz, sc-166210), Plk1 (Abcam, ab12157) and β-galactosidase (CST, #27198) were incubated with the membranes at 4 °C overnight, followed by incubation with the respective secondary antibodies.

Techniques: Over Expression, Knockdown, Staining, Colony Assay, Expressing